ChIP (Chromatin Immunoprecipitation)
نویسندگان
چکیده
منابع مشابه
Chromatin immunoprecipitation (ChIP).
Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with post-translational modifications at specific genomic l...
متن کاملChromatin Immunoprecipitation (ChIP) using Drosophila tissue.
Epigenetics remains a rapidly developing field that studies how the chromatin state contributes to differential gene expression in distinct cell types at different developmental stages. Epigenetic regulation contributes to a broad spectrum of biological processes, including cellular differentiation during embryonic development and homeostasis in adulthood. A critical strategy in epigenetic stud...
متن کاملChromatin Immunoprecipitation-based Sequencing (ChIP-Seq) on the SOLiD System
SoliD System chip-Seq Analysis The SOLiD System’s ability to generate over 400 million sequence tags (35 bp sequence reads) in a single run enables whole genome ChIP analysis of complex organisms. Sequence tags are mapped to a reference sequence and counted, to identify specific regions of protein binding. The ultra high throughput of the system provides researchers with the sensitivity and the...
متن کاملChromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
Signaling pathways regulate gene expression programs via the modulation of the chromatin structure at different levels, such as by post-translational modifications (PTMs) of histone tails, the exchange of canonical histones with histone variants, and nucleosome eviction. Such regulation requires the binding of signal-sensitive transcription factors (TFs) that recruit chromatin-modifying enzymes...
متن کاملChromatin immunoprecipitation (ChIP) scanning identifies primary glucocorticoid receptor target genes.
The global physiological effects of glucocorticoids are well established, and the framework of transcriptional regulation by the glucocorticoid receptor (GR) has been described. However, the genes directly under GR control that trigger these physiological effects are largely unknown. To address this issue in a single cell type, we identified glucocorticoid-responsive genes in A549 human lung ad...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2019
ISSN: 2043-0116
DOI: 10.1038/protex.2018.143